5 Simple Statements About flow cytometers Explained
5 Simple Statements About flow cytometers Explained
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Information-enriched characterization of mobile populations. Morphological information and facts from illustrations or photos can increase to your richness of flow cytometry info. For example, the determine demonstrates an or else typical apoptosis assay working with Annexin V and PI, incorporating mobile imaging to characterize cells in each inhabitants to reveal morphologically unique capabilities. These insights couldn't are already attained from multiplex staining alone.
As samples are obtained around the Attune CytPix Flow Cytometer, the substantial-speed brightfield camera captures and suppliers illustrations or photos of detected occasions in a amount approximately 6,000 illustrations or photos for every second, depending on the flow level and impression measurement. For bigger versatility, Attune Cytometric Software program permits you to modify the graphic seize frequency as desired and select a certain gate for graphic capture, if demanded.
Combine acoustic concentrating technology with higher-parameter Investigation for flexible spectral unmixing and traditional workflows.
Discover new insights into your experimental details by visualizing populations utilizing put together fluorescence and morphology information. Our computer software interface wizards empower downsampling, concatenation (merged documents) and dimensionality tools that are developed into your program for an intuitive, streamlined information Investigation expertise.
"An image is just not quite value a thousand dots, but it really definitely helps you to see pictures. It is great to determine illustrations or photos that correlate with staining. Such as, AnnexinV-positive cells that happen to be morphologically distinct than wholesome cells, or CD14+ monocytes that are larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Below, a highly trained consumer has gated singlets confidently. After assessing the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate has a lot more than four% aggregates.
Pair your Attune flow cytometer using an autosampler for elevated workflow performance, and insert robotic integration for max throughput.
Dependable picture high quality even at substantial throughput Acoustic focusing and a higher-pace camera Mix to graphic these T cells continually at small or significant flow fees. Effortlessly change aim and digital camera configurations to meet experimental specifications.
Early indications of this contamination had initially been dismissed as particles. Backgating to find out new read more populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also helps you to use morphological functions to find out fascinating subpopulations that may not be clear from flow cytometry data on your own.
Possibly most importantly, these events have cells of Obviously unique phenotypes which may lead to incorrect conclusions about double favourable activities (specifically in unusual populations).
Audio waves enable specific mobile alignment, resulting in Remarkable data top quality and ~10X speedier sample flow premiums.
We Earlier shown the strength of imaging Auto-T/Ramos mobile interactions. Allow’s take a look at just the population of biggest curiosity, the double good activities, to learn more. We could now use prolonged morphological parameters (here, circularity and skewness of depth) to even further examine the characteristics of those populations and refine gating on these situations, expanding information robustness.
Leverage the power of Sophisticated technology and innovation to speed up your discoveries. Have faith in from the Attune Flow Cytometer relatives to provide the dependability, pace, and accuracy that set your perform apart.
Adaptable payment approach from Agilent Monetary Methods assist you to have the critical instrumentation you may need for fast challenge, or to improve your lab.
Gating on these prolonged parameters allows you to speedily and precisely determine populations of curiosity and confirm gating tactic with little if any guide critique. The Attune application features access to state-of-the-artwork machine Mastering Assessment instruments inside an expertly built user interface, which makes it exceptionally easy to use.
The Attune Xenith cytometer is actually a significant-parameter spectral analyzer, giving equally spectral unmixing and traditional flow capabilities for in depth and functional mobile Investigation.
Agilent Price Assure assures you at least a decade of instrument use in the date you buy. Or else, we will credit rating you While using the residual worth of the procedure toward an upgraded design.
Listed here, we present that by gating approaches applying picture-primarily based quantitative parameters can distinguish interacting cells from coincident occasions to much more precisely assess interacting cells.